Web supplement to
"Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe"

Dong-Uk Kim1,14, Jacqueline Hayles2,14, Dongsup Kim3,14, Valerie Wood2,4,14, Han-Oh Park5,14, Misun Won1,14, Hyang-Sook Yoo1,14, Trevor Duhig2, Miyoung Nam1, Georgia Palmer2, Sangjo Han3, Linda Jeffery2, Seung-Tae Baek1, Hyemi Lee1, Young Sam Shim1, Minho Lee3, Lila Kim1, Kyung-Sun Heo1, Eun Joo Noh1, Ah-Reum Lee1, Young-Joo Jang1, Kyung-Sook Chung1, Shin-Jung Choi1, Jo-Young Park1, Youngwoo Park1, Hwan Mook Kim6, Song-Kyu Park6, Hae-Joon Park5, Eun-Jung Kang5, Hyong Bai Kim7, Hyun-Sam Kang8, Hee-Moon Park9, Kyunghoon Kim10, Kiwon Song11, Kyung Bin Song12, Paul Nurse2,13 and Kwang-Lae Hoe1,6
1Integrative Omics Research Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea 2Cancer Research UK, The London Research Institute, 44, Lincoln's Inn Fields, LondonWC2A 3PX, UK 3Department of Bio and Brain Engineering, Korea Advanced Institute of Science & Technology (KAIST), Yuseong, Daejeon, Korea 4Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK 5Bioneer Corporation, Daejeon, Korea 6Bio-Evaluation Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon, Korea 7Department of Bioinformatics & Biotechnology, Korea University, Jochiwon, Chungnam, Korea 8School of Biological Sciences, Seoul National University, Seoul, Korea 9Department of Microbiology, Chungnam National University, Yuseong, Daejeon, Korea 10Division of Life Sciences, Kangwon National University, Chuncheon, Kangwon, Korea 11Department of Biochemistry, Yonsei University, Seoul, Korea 12Department of Food and Nutrition, Chungnam National University, Yuseong, Daejeon, Korea 13The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA 14These authors contributed equally to this work.

Correspondence and requests for material should be addressed to K.H. (e-mail: kwanghoe@kribb.re.kr) or P.N. (e-mail: nurse@mail.rockefeller.edu).



We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome. This resource provides a powerful tool for biotechnological and eukaryotic cell biology research. Comprehensive gene dispensability comparisons with budding yeast, the first time such studies have been possible between two eukaryotes, revealed that 83% of single copy orthologues in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than non-essential genes to be single copy and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.

Inquiries can be addressed to kwanghoe@kribb.re.kr.